One-carbon, carnitine, and glutathione metabolism-related biomarkers in peripartal Holstein cows are altered by prepartal body condition

We investigated how prepartal body condition score (BCS) alters key hepatic enzymes associated with 1-carbon, carnitine, and glutathione metabolism the related biomarkers in liver tissue plasma of periparturient dairy cows. Twenty-six multiparous Holstein cows were retrospectively selected according to BCS at 4 wk prepartum divided into high (HighBCS, ≥ 3.50) normal (NormBCS, ≤ 3.25) groups (n = 13 each). Blood samples obtained −30, −10, 7, 15, 30 d relative calving. Liver biopsies performed −15, calving, used assess protein abundance via Western blot assay. Cows HighBCS group lost ∼1 unit between −4 around while NormBCS ∼0.5 same period. Prepartal dry matter intake (DMI, kg/d) did not differ groups. Compared cows, had higher postpartal DMI milk yield (+5.34 kg/d). In addition, greater overall concentrations fatty acids activity neutrophil-enriched enzyme myeloperoxidase observed compared Despite similar reactive oxygen metabolite both d, lower β-carotene tocopherol, explaining (BCS × Time) antioxidant capacity (ferric reducing ability plasma). The also malondialdehyde superoxide dismutase d. Overall, 1-carbon cystathionine-β-synthase, betaine-homocysteine methyltransferase, methionine adenosyltransferase 1 A (MAT1A), as well metabolism-related S-transferase α peroxidase 3 (GPX3). mu (GSTM1) −15 7 was observed. Regardless BCS, increased GSTM1 GPX3 marked decrease gamma-butyrobetaine dioxygenase from −10 suggested a de novo carnitine synthesis that partly explained by MAT1A. data suggest biologic links before yield, immune response, reactions encompassing metabolism, synthesis.